Inhaled nitric oxide reduces ischemia-reperfusion injury in rat lungs from non–heart-beating donors

摘要: Objective If lungs could be retrieved from non–heart-beating donors, the critical shortage of for transplantation alleviated. However, subjected to warm ischemia develop edema when reperfused. We hypothesized that ventilation rat donors with nitric oxide during period alone, reperfusion, or both might reduce ischemia-reperfusion injury. Methods An isolated perfused lung model measured filtration coefficient and accumulation water by wet/dry weight ratio. Donor rats were euthanized, then immediately after death 2 3 hours postmortem. Lungs postmortem either not ventilated 100% oxygen alone 40 ppm in oxygen. In circuit, alveolar gas without oxide. Results Nitric administration donor perfusion circuit reduced treated on reperfusion had coefficients ratios similar those death. was most beneficial administered at associated increased levels cyclic guanosine monophosphate, determined enzyme-linked immunosorbent assay. Conclusions Administration facilitate reducing injury capillary leak.