Isolation and amino acid compositions of four somatostatin-like substances in chicken hypothalamic extract.
作者： YOSHIHISA HASEGAWA , KAORU MIYAMOTO , MITSUO NOMURA , MASAO IGARASHI , KENJI KANGAWA
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摘要: Four peptides having SRIF-like bioactivity when assayed by a rat anterior pituitary cell culture system have been isolated from chicken hypothalamic extracts. These four purified 2,000-5,000 hypothalami in yield of 2.2 nmol to 16 nmol. The SRIFs characterized amino acid analysis. One consists 28 acids which are identical with mammalian SRIF-28 except that one Met residue is replaced Leu residue. Three other substances low mol wt also pure state. SRIF-14, as judged chromatographic comparison on HPLC and its composition well the specific cells. another but it different SRIF-14 elution position HPLC. third substance less potent than SRIF-14. It concluded several exist SRIF-28, substitution Leu, one, major components hypothalamus.
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