High-performance liquid chromatography for assaying NAD glycohydrolase from Neurospora crassa conidia
作者： Piergiorgio Pietta , Mario Pace , Faustino Menegus
DOI: 10.1016/0003-2697(83)90211-7
关键词:
摘要: A rapid and sensitive high-performance liquid chromatographic technique was developed to determinate NAD glycohydrolase (EC 3.2.2.5.) activity from Neurospora crassa conidia. The separation of the assay substrate products achieved by isocratic reverse-phase chromatography peaks were detected absorbance at 259 nm. Quantities NAD+ nicotinamide as small 10 pmol could be measured.
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