Evaluation of hexokinase gene expression in colorectal cancer using bioinformatics tools

摘要: Disruption of energy metabolism that mainly involves activation glycolysis occurs even at the early stages cancer due to, for example, overexpression hexokinases such as HK1 and HK2, most part represents a universal feature malignant tumor formation. Overexpression which is considered marker poor prognosis, commonly believed to take place in colorectal cancer, third mortality morbidity among cases worldwide. The RNA-sequencing data Cancer Genome Atlas project (the largest resource area molecular oncology, contains genomic, transcriptomic, epigenomic thousands samples over 20 forms cancer) were analyzed using CrossHub software was developed by authors, which, on contrary, revealed prevalence reduced levels HK2 mRNA only slight changes expression gene. transcriptome analysis (283 41 normal tissue samples) agreement with results following experimental evaluation hexokinase gene quantitative polymerase chain reaction. Increased genes observed minor samples: 12% 30% HK2. At same time decreased level found 50% cases. Correlation showed between (Spearman’s rank correlation coefficient rS = 0.43, p < 0.001), can be explained common deregulation mechanisms these tumors. HK3 increased significantly 60% samples; it likely precisely 3 contributed cancer.