Biochemical signatures of in vitro radiation response in human lung, breast and prostate tumour cells observed with Raman spectroscopy.

摘要: This work applies noninvasive single-cell Raman spectroscopy (RS) and principal component analysis (PCA) to analyze correlate radiation-induced biochemical changes in a panel of human tumour cell lines that vary by tissue origin, p53 status intrinsic radiosensitivity. Six lines, derived from prostate (DU145, PC3 LNCaP), breast (MDA-MB-231 MCF7) lung (H460), were irradiated vitro with single fractions (15, 30 or 50 Gy) 6 MV photons. Remaining live cells harvested for RS at 0, 24, 48 72 h post-irradiation, along unirradiated controls. Single-cell spectra acquired 20 per sample utilizing 785 nm excitation laser. All (200 line) individually post-processed using established methods the total data set each line was analyzed PCA standard algorithms. One detected identification statistically significant score distributions samples, as compared first 24–72 post-irradiation. These response signatures arise cellular concentrations aromatic amino acids, conformational protein structures certain nucleic acid lipid functional groups. Correlation between components separates into three distinct categories: R1 (H460 MCF7), R2 PC3) R3 (DU145 LNCaP). categories partially segregate according radiosensitivity, are radioresistant (SF2 > 0.6) radiosensitive < 0.5). The further gene status, corroborated cycle Potential mechanisms underlying our observations proposed, such (1) regulated synthesis degradation structured proteins (2) expression anti-apoptosis factors other survival signals. study demonstrates utility radiobiological radiation response, indicates potential future studies designed investigate, monitor predict response.