The turnover of basal lamina glycosaminoglycan correlates with epithelial morphogenesis

摘要: Abstract The mouse embryonic submandibular epithelium begins as a single bud from the floor of mouth which, under influence its surrounding mesenchyme, grows and forms lobules that subsequently branch repetitively. lobular morphology 13-day is maintained by basal lamina which continuous layer on interlobular clefts but interrupted distal aspects lobules. structural integrity this dependent upon glycosaminoglycan (GAG) histochemistry, more abundant than We have investigated basis for these regional differences in examining synthesis degradation total GAG well accumulation loss laminar during morphogenesis gland. Autoradiography histochemistry show rapidly turning over. Although it relatively stable clefts, degraded This difference can account variation accumulation. results incorporation kinetics precursor pool specific activities epithelial rate greater base lobules, includes clefts. In contrast, morphogenesis, becomes replacement stalk appears to be steady state regarding metabolism. propose (a) rapid produces interruptions lamina, allowing growth expansion, (b) metabolic stability maintains serves cellular constraint. are consistent with model mesenchyme remodels turn, dictates morphology. Regulation turnover may general mechanism controlling behavior cell populations.