Autologous tolerogenic dendritic cells derived from monocytes of systemic lupus erythematosus patients and healthy donors show a stable and immunosuppressive phenotype.

摘要: Systemic lupus erythematosus (SLE) is an autoimmune disease with unrestrained T-cell and B-cell activity towards self-antigens. Evidence shows that apoptotic cells (ApoCells) trigger autoreactive response against nuclear antigens in susceptible individuals. In this study, we focus on generating characterizing tolerogenic dendritic (tolDCs) to restore tolerance ApoCells. Monocyte-derived (DCs) from healthy controls patients SLE were treated dexamethasone rosiglitazone induce tolDCs. Autologous lymphocytes generated by UV irradiation given tolDCs as a source of Lipopolysaccharide (LPS) was used maturation stimulus the expression co-stimulatory molecules secretion cytokines. TolDCs showed reduced after LPS stimulation compared mature DCs. The same phenomenon observed ApoCells LPS. addition, ApoCell-loaded stimulated secreted lower levels interleukin-6 (IL-6) IL-12p70 than DCs without differences IL-10 secretion. functionality assessed their capacity prime allogeneic T cells. displayed suppressor properties demonstrated significantly proliferation activation. monocytes have stable immature/tolerogenic phenotype can modulate CD4+ These make them suitable for antigen-specific immunotherapy SLE.