The hemagglutinin envelope protein of canine distemper virus (CDV) confers cell tropism as illustrated by CDV and measles virus complementation analysis.

摘要: Measles virus (MV) and canine distemper (CDV) are morbilliviruses that cause acute illnesses several persistent central nervous system infections in humans dogs, respectively. Characteristically, the cytopathic effect of these viruses is formation syncytia permissive cells. In this study, a vaccinia expression was used to express MV CDV hemagglutinin (HA) fusion (F) envelope proteins. We found cotransfecting F HA genes or resulted extensive syncytium cells while transfecting either alone did not. Similar experiments with heterologous pairs proteins, CDV-F MV-HA MV-F CDV-HA, caused significant cell both cases. These results indicate system, requires HA; however, functions proteins interchangeable between two types morbilliviruses. Human-mouse somatic hybrids were determine human chromosome conferring susceptibility CDV. Of 12 screened, none sensitive MV. Two containing 19 formed following infection. addition, underwent when cotransfected CDV-HA (but not MV-HA) glycoproteins by using system. To discover viral component responsible for specificity, complementation coexpressing CDV-sensitive performed. only presence CDV-HA. support idea protein tropism. Furthermore, necessary process, it from other