Somatic Embryogenesis and Plant Regeneration from Suspension Callus Culture in Melon (Cucumis melo L.)
作者： Toshiro ORIDATE , Katsuji OOSAWA
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摘要: Somatic embryos and plantlets were induced from suspension culture of cotyledon callus in melon (Cucumis melo L.). Cotyledons excised directly bared mature seed cultured on MS agar media with several concentrations 2, 4-D BA. Three weeks old calli transferred to liquid medium without growth regulators, a reciprocal gyratory shaker (115 rpm). observed within 2-3 the media. The 1.0mg/l 4-D+0.1mg/l BA most suitable for embryogenesis.
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