Fluorescence imaging of interscapular brown adipose tissue in living mice

摘要: Brown adipose tissue (BAT) plays a key role in energy expenditure and heat generation is promising target for diagnosing treating obesity, diabetes related metabolism disorders. While several nuclear magnetic resonance imaging methods are established detecting human BAT, there no convenient protocols high throughput of BAT small animal models. Here we disclose simple but effective method non-invasive optical interscapular mice using micellar formulation the commercially available deep-red fluorescent probe, SRFluor680. Whole-body fluorescence living shows extensive accumulation probe ex vivo analysis 3.5-fold selectivity over WAT. Additional studies indicate that SRFluor680 uptake independent mouse species metabolic state. The results consistent with an unusual pharmacokinetic process involves irreversible translocation lipophilic from micelle nanocarrier into adipocytes within BAT. Multimodal PET/CT planar fluorescence/X-ray same co-localization mass signal reported by PET agent, 18F-FDG. path towards new, dual molecular paradigm allows separate visualization subject.