Multifunction Protein Staphylococcal Nuclease Domain Containing 1 (SND1) Promotes Tumor Angiogenesis in Human Hepatocellular Carcinoma through Novel Pathway That Involves Nuclear Factor κB and miR-221

摘要: Staphylococcal nuclease domain-containing 1 (SND1) is a multifunctional protein that overexpressed in multiple cancers, including hepatocellular carcinoma (HCC). Stable overexpression of SND1 Hep3B cells expressing low level augments, whereas stable knockdown QGY-7703 high inhibits establishment xenografts nude mice, indicating promotes an aggressive tumorigenic phenotype. In this study we analyzed the role regulating tumor angiogenesis, hallmark cancer. Conditioned medium from Hep3B-SND1 stably overexpressing augmented, QGY-SND1si siRNA significantly inhibited as by chicken chorioallantoic membrane assay and human umbilical vein endothelial cell differentiation assay. We unraveled linear pathway which SND1-induced activation NF-κB resulted induction miR-221 subsequent angiogenic factors Angiogenin CXCL16. Inhibition either these components significant inhibition thus highlighting importance molecular cascade function. Because regulates miR-221, two important determinants HCC controlling phenotype, might be effective strategy to counteract fatal malady.