Androgens stimulate coordinated lipogenic gene expression in normal target tissues in vivo

摘要: In prostate cancer cell lines in culture androgens cause a marked and coordinated upregulation of the expression several lipogenic genes. Here, using castrated male Wistar rats as an experimental paradigm, we investigated whether androgen stimulation gene represents more general physiological regulation non-cancerous androgen-responsive cells well. typical target tissues for action such ventral lacrimal gland, deprivation resulted reduction mRNA protein levels genes involved fatty acid (fatty synthase acetyl-CoA-carboxylase) cholesterol synthesis (HMG-CoA-reductase farnesyl diphosphate synthase). Readministration testosterone immediately following orchidectomy restored all four Substitution by non-aromatizable dihydrotestosterone gave rise to comparable changes under investigation, confirming involvement receptor observed effects. support coordinate nature this regulation, androgen-induced is accompanied increase nuclear content SREBP, key transcription factor. Taken together, these findings provide evidence not only but also vivo.