Effects of icariin on long noncoding RNA and mRNA expression profile in the aortas of apoE-deficient mice.

摘要: Objective: The beneficial effects of icariin (ICA) in ameliorating atherosclerosis (AS) are well known, but the underlying protective mechanism has not been fully elucidated. present study aimed to investigate altered long noncosing RNA (lncRNA) and mRNA expression profiles ApoE−/− mice after ICA treatment. Method: atherosclerotic plaque area was evaluated on high-fat diet (HFD)-induced treated with either or vehicle. LncRNA integrated microarrays performed aortic tissues. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis were utilized explore significant function differentially expressed (DE) mRNAs, global signal transduction network constructed select key lncRNA–mRNA co-expression built find out interactions between lncRNA mRNA. Quantitative real-time PCR (qPCR) used further validate expressions selected lncRNAs mRNAs. Results: Administration significantly reduced size 12 weeks (P<0.05). A total 1512 DE 2059 mRNAs identified. mRNAs: protein kinase C, β (Prkcb), Cyp2c65, Mapk10, Calmodulin 5 (Calm5), Calmodulin-like 3 (Calml3) Camk4 as hub correlated identified important regulatory lncRNAs. target pairs such lncRNA-NONMMUT000659/Prkcb may play critical roles AS development mediated by ICA. Conclusion: Taken together, our highlights a panel that could explain molecular ICA’s anti-atherosclerotic effects. work lays foundation for subsequent genes functional researches, which contribute provide new therapeutic targets AS.