Development of an in vitro, isolated, infected spore testing model for disinfectant testing of Microsporum canis isolates.

摘要: The isolated infected hair model is a commonly used technique to test the fungicidal efficacy of topical therapies against Microsporum canis. most uses mats infective hairs, and results from various laboratories have differed. objectives this study were develop method produce spores for testing when only mycelial forms available semiquantitative that not pooled samples testing. Ten isolates M. canis in study. Juvenile guinea pigs easily using large numbers harvested Eight dilutions disinfectants tested. Fungal culture data evaluated an endpoint dilution at which there was 100% activity, i.e. no growth on plates. 10 showed identical results. Chlorhexidine Virkon(R) S ineffective even x4 manufacturer's recommended dilution. Lime sulphur (1 : 33), enilconazole (20 microL mL(-1)), bleach 10) consistently effective In addition, lime concentration diluted 1 4 or as dilute recommended.