Pronounced Effect of DNA Hybridization on Click Reaction Efficiency
作者： Christian T. Wirges , Philipp M. E. Gramlich , Katrin Gutsmiedl , Johannes Gierlich , Glenn A. Burley
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摘要: We report about the efficiency of click reaction directly on PCR products containing alkyne-modified uridine nucleosides. Galactose azide was clicked onto single-stranded and double-stranded 300mer DNA in order to investigate sugar labeling under various conditions. Single-stranded prepared by selective lambda exonuclease digestion 5′-phosphate-labeled double strands. A comparison modified single stranded 300mers identical conditions shows a pronounced effect strandedness yield reaction.
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