Copper-Catalyzed Azide–Alkyne Click Chemistry for Bioconjugation
作者： Stanislav I. Presolski , Vu Phong Hong , M.G. Finn
DOI: 10.1002/9780470559277.CH110148
关键词: Alkyne 、 Azide 、 Ligand 、 Combinatorial chemistry 、 Bioconjugation 、 Chemistry 、 Cycloaddition 、 Reagent 、 Click chemistry 、 Reducing agent
摘要: The copper‐catalyzed azide‐alkyne cycloaddition reaction is widely used for the connection of molecular entities of all sizes. A protocol is provided here for the process with biomolecules. Ascorbate is used as reducing agent to maintain the required cuprous oxidation state. Since these convenient conditions produce reactive oxygen species, five equivalents of a copper‐binding ligand are used with respect to metal. The ligand both accelerates the reaction and serves as a sacrificial reductant, protecting the biomolecules …
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