Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne‐Bearing Triphosphates and Click Chemistry

摘要: We report the combination of "click chemistry" with PCR by using alkyne-modified triphosphates for efficient and homogeneous labeling DNA. A series modified products different lengths (300, 900, 2000 base pairs) were prepared a variety alkyne- azide-containing polymerases. After intensive screening real-time methods, protocols developed that allow amplification genes these similar efficiency to standard PCR. The click reaction on highly fragments provided conversion rates above 90 % resulted in functionalization hundreds alkynes large DNA superb selectivity efficiency.