Patient tumor EGFR and PDGFRA gene amplifications retained in an invasive intracranial xenograft model of glioblastoma multiforme.

摘要: We have previously described a panel of serially transplantable glioblastoma multiforme xenograft lines established by direct subcutaneous injection patient tumor tissue in the flanks nude mice. Here we report characterization four these with respect to their histopathologic, genetic, and growth properties following heterotopic-to-orthotopic (flank-to-intracranial) transfer. Cells from short-term cultures, excised flank xenografts, were harvested injected into brains mice (106 cells per injection). The intracranial tumors generated injections all highly mitotic as well invasive, but they lacked necrotic features most instances failed show endothelial cell proliferation instances. For receiving common explant culture, rate was consistent, indicated relatively narrow ranges survival time. In contrast loss epidermal factor receptor gene (EGFR) amplification high-level overexpression EGFR retained two EGFR-amplified tumors. A third expression platelet-derived alpha gene. Because transfer propagation preserves tyrosine kinase (RTK) tumors, this approach should facilitate investigations for determining extent which RTK status influences response RTK-directed therapies. fact that such studies carried out using an invasive model anatomically appropriate context ensure rigorous preclinical assessment agent efficacy.