Ability of Commercial Demineralized Freeze-Dried Bone Allograft to Induce New Bone Formation

摘要: Demineralized freeze-dried bone allograft (DFDBA) has been used extensively in periodontal therapy. The rationale for use of DFDBA includes the fact that proteins capable inducing new bone; i.e., morphogenetic proteins, can be isolated from grafts. Commercial banks have provided to dental practitioner many years; however, these organizations not verified osteoinductive capacity their preparations. aim this study was determine ability commercial preparations induce formation. with particle sizes ranging 200 500 microns received six using various production methods. Different lots same tissue bank were sometimes available. A total 14 examined. surface area particles each sample measured morphometrically and pH a solution containing after suspension distilled water determined. Samples lot implanted intramuscularly (10 mg) or subcutaneously (20 into three different animals biopsies harvested 4 weeks. One 8 At harvest, where had excised examined by light microscopy. produce evaluated amount residual measured. results show all variety shapes sizes, both before implantation 1 2 months implantation. suspensions also varied between batches, as well banks. None induced formation when subcutaneously. Intramuscular implants months. two caused only However, one did at all. Particle size correlated correlate bone. differs formation, but are related. indicates wide variation exist varies widely. Furthermore, suggest methods assays evaluating should developed standardized.