Effector cell mediated cytotoxicity measured by intracellular Granzyme B release in HIV infected subjects.

摘要: CD8+ cytotoxic T lymphocyte (CTL) activity is currently believed to be one of the key immunologic mechanisms responsible for prevention or attenuation HIV-1 infection. The induction cell activation may also result in production soluble non-classical lytic factors that are associated with protection from infection slower disease progression. Traditionally, CTL responses have been measured by classic chromium release assay, monitoring ability cells (Effector cells) lyse radiolabelled HLA — matched “target cells“ express appropriate antigen-MHC complex. This method not only labor intensive, semi quantitative assay at best, but needs fresh, non-cryopreserved cells. Recently, cytokine specific ELISPOT assays tetrameric MHC-I/ peptide complexes utilized directly quantitate circulating effector cells, and these more sensitive, reproducible than traditional lysis can performed on cryopreserved Although assessment antiviral secreted activated populations they extremely expensive perform. We used FACS Analysis measure Granzyme B as a function mediated cytotoxicity. helps identifies phenotype elucidating this immune response. described monitors immunological response simple perform, precise time efficient ideal screening large number samples.