Changes in uterine mast cells during the estrous cycle in the Syrian hamster.

摘要: Adult virgin female Syrian hamsters were killed at known stages of the estrous cycle, and mast cells counted in myometrium, endometrium, mesometrial-triangle region uterus pinna. Tissues fixed Helly's solution, stained using 0.06% toluidine blue 0.12 M Michaelis' veronal acetate-hydrochloric acid buffer, pH 4.5. The number myometrium endometrium was found to vary with whereas pinna showed no such variation. lowest on day 4 cycle (the before night during which ovulation would occur) increased approximately twofold maximal levels days 1 2. Intermediate seen 3. origin increase cell numbers from investigated a combined alcian blue--safranin stain differentiate between immature mature mast-cell granules. results obtained did not support hypothesis that due de novo differentiation precursors, but, equally, evidence refute this hypothesis.