Improved Electrochemical Detection of Purine Nucleobases at Mechanically Roughened Edge‐Plane Pyrolytic Graphite Electrode
作者： Stanislav Hasoň , Lukáš Fojt , Peter Šebest , Miroslav Fojta
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摘要: Mechanically grinded edge-plane pyrolytic graphite electrode (g-PGEe) was applied in voltammetric analysis of purine nucleobases, acid-hydrolyzed synthetic oligodeoxynucleotides and a nonhydrolyzed plasmid DNA. Properties the mechanically grinded electrodes these analytical applications were compared with some other carbon electrode types. We show that the surface grinding 15-mu M SiC particles resulted remarkable improvement oxidation signals of purine bases no addition copper ions. Addition the copper ions., causing strong enhancement purine oxidation responses at fine-polished electrodes, had only small effect on purine g-PGEe. On the other hand, g-PGEe appeared less suitable for ex situ AdTS measurements DNA, compared to freshly peeled basal plane graphite electrode.
sci-hub.se 参考文章(30)
Emil Palecek, Miroslav Fojta, Electrochemical DNA Sensors John Wiley & Sons, Ltd. pp. 127- 192 ,(2005) , 10.1002/352760376X.CH5
František Jelen, Bogdan Yosypchuk, Alena Kourilová, Ladislav Novotný, Emil Paleček, Label-free determination of picogram quantities of DNA by stripping voltammetry with solid copper amalgam or mercury electrodes in the presence of copper. Analytical Chemistry. ,vol. 74, pp. 4788- 4793 ,(2002) , 10.1021/AC0200771
Christopher MA Brett, Ana Maria Oliveira Brett, Silvia HP Serrano, None, On the adsorption and electrochemical oxidation of DNA at glassy carbon electrodes Journal of Electroanalytical Chemistry. ,vol. 366, pp. 225- 231 ,(1994) , 10.1016/0022-0728(93)02994-S
Christie D. Allred, Richard L. McCreery, Adsorption of catechols on fractured glassy carbon electrode surfaces Analytical Chemistry. ,vol. 64, pp. 444- 448 ,(1992) , 10.1021/AC00028A020
S HASON, V VETTERL, Microanalysis of oligodeoxynucleotides by cathodic stripping voltammetry at amalgam-alloy surfaces in the presence of copper ions. Talanta. ,vol. 69, pp. 572- 580 ,(2006) , 10.1016/J.TALANTA.2005.10.029
Xiaohua Cai, Gustavo Rivas, Percio AM Farias, Haruki Shiraishi, Joseph Wang, Miroslav Fojta, Emil Paleček, None, Trace measurements of plasmid DNAs by adsorptive stripping potentiometry at carbon paste electrodes Bioelectrochemistry and Bioenergetics. ,vol. 40, pp. 41- 47 ,(1996) , 10.1016/0302-4598(95)05048-5
Jpseph Wang, Gustavo Rivas, Xiaohua Cai, Manuel Chicharro, Narasaiah Dontha, Denbai Luo, Emil Palecek, Pater E. Nielsen, Adsorption and detection of peptide nucleic acids at carbon paste eletrodes Electroanalysis. ,vol. 9, pp. 120- 124 ,(1997) , 10.1002/ELAN.1140090206
Lukáš Fojt, Stanislav Hasoň, Sensitive determination of oligodeoxynucleotides by anodic adsorptive stripping voltammetry at surface-roughened glassy carbon electrode in the presence of copper Journal of Electroanalytical Chemistry. ,vol. 586, pp. 136- 143 ,(2006) , 10.1016/J.JELECHEM.2005.07.027
Joseph Wang, Abdel-Nasser Kawde, Amplified label-free electrical detection of DNA hybridization Analyst. ,vol. 127, pp. 383- 386 ,(2002) , 10.1039/B110821M
Stanislav Hasoň, Vladimír Vetterl, František Jelen, Miroslav Fojta, Improved sensitivity and selectivity of uric acid voltammetric sensing with mechanically grinded carbon/graphite electrodes Electrochimica Acta. ,vol. 54, pp. 1864- 1873 ,(2009) , 10.1016/J.ELECTACTA.2008.10.020