Recombinant nematode anticoagulant protein c2 inhibits cell invasion by decreasing uPA expression in NSCLC cells

摘要: Nematode anticoagulant protein c2 (NAPc2) is an 85-residue polypeptide originally isolated from the hema - tophagous hookworm, Ancylostoma caninum. Several studies have shown that rNAPc2 inhibits growth of primary and metastatic tumors in mice independently its ability to initiate coagulation. We obtained bioactive recombinant by splicing rNAPc2-intein-CBD fusion proteins expressed E. coli ER2566. In vitro assay, obviously inhibited invasive non-small cell lung cancer (NSCLC) cells a dose-dependent manner. Furthermore, suppressed tumor vivo daily intraperito- neal injection NSCLC xenograft model nude mice. Respectively, downregulated produc- tion urokinase plasminogen activator (uPA) (P<0.05) nuclear factor-κB (NF-κB) activity. also identi- fied inhibition NF- κB activity impaired invasion reduced uPA production cells. Meanwhile, NF-κB was found directly bind promoter vitro. These results demonstrated at least part through downregulation κB- dependent metastasis-related gene expression NSCLC. Our suggest uPA, known metastasis-promoting gene, indirectly regulated activa- tion. indicate may be potent agent for prevention progression.