OpenWorkstation: A modular open-source technology for automated in vitro workflows
作者： Sebastian Eggert , Pawel Mieszczanek , Christoph Meinert , Dietmar W Hutmacher
DOI: 10.1016/J.OHX.2020.E00152
关键词:
摘要: Automation liberates scientific staff from repetitive tasks, decreases the probability of human error and consequently enhances reproducibility lab experiments. However, use laboratory automation in academic laboratories is limited due to high acquisition costs inability customize off-the-shelf hardware. To address these challenges, we present an Open Source Hardware concept, referred as OpenWorkstation, build assembly line-inspired platform consisting ready-to-use customizable modules. In contrast current standalone solutions, OpenWorkstation concept enables combination single hardware modules – each with a specific set functionalities modular workstation provide fully automated setup. The base setup consists pipetting transport module designed execute basic protocol steps for vitro research applications, including operations liquids viscous substances transportation cell culture vessels between We demonstrate successful application this within case study by development storage facilitate high-throughput studies photo-crosslinker initiate photo-induced polymerization hydrogel solutions. Systems Engineering framework customized development, guidance design presented modules, operational instructions on usage workstation. By combining capabilities various open source instrumentations into technology platform, will efficient reliable experimentation research. Ultimately, allow groups improve replicability process towards more economical innovative future.
uq.edu.au参考文章(61)
Joshua M. Pearce, Introduction to Open-Source Hardware for Science Open-Source Lab#R##N#How to Build Your Own Hardware and Reduce Research Costs. pp. 1- 11 ,(2014) , 10.1016/B978-0-12-410462-4.00001-9
Ignacio Arganda-Carreras, Carlos OS Sorzano, Roberto Marabini, José María Carazo, Carlos Ortiz-de-Solorzano, Jan Kybic, None, Consistent and Elastic Registration of Histological Sections Using Vector-Spline Regularization Computer Vision Approaches to Medical Image Analysis. pp. 85- 95 ,(2006) , 10.1007/11889762_8
Tetuko Kurniawan, Karl T Ulrich, Steven D Eppinger, Product Design and Development ,(1995)
Stephen V. Frye, Michelle R. Arkin, Cheryl H. Arrowsmith, P. Jeffrey Conn, Marcie A. Glicksman, Emily A. Hull-Ryde, Barbara S. Slusher, Tackling reproducibility in academic preclinical drug discovery Nature Reviews Drug Discovery. ,vol. 14, pp. 733- 734 ,(2015) , 10.1038/NRD4737
Aaron Enten, Yujia Yang, Zihan Ye, Ryan Chu, Tam Van, Ben Rothschild, Francisco Gonzalez, Todd Sulchek, A Liquid-Handling Robot for Automated Attachment of Biomolecules to Microbeads. Journal of Laboratory Automation. ,vol. 21, pp. 526- 532 ,(2016) , 10.1177/2211068215601846
Bas Wijnen, Emily J. Hunt, Gerald C. Anzalone, Joshua M. Pearce, Open-Source Syringe Pump Library PLoS ONE. ,vol. 9, pp. e107216- 8 ,(2014) , 10.1371/JOURNAL.PONE.0107216
J. M. Pearce, Building Research Equipment with Free, Open-Source Hardware Science. ,vol. 337, pp. 1303- 1304 ,(2012) , 10.1126/SCIENCE.1228183
Nathan Blow, Lab automation: tales along the road to automation. Nature Methods. ,vol. 5, pp. 109- 112 ,(2008) , 10.1038/NMETH0108-109
Michael Woelfle, Piero Olliaro, Matthew H. Todd, Open science is a research accelerator Nature Chemistry. ,vol. 3, pp. 745- 748 ,(2011) , 10.1038/NCHEM.1149
Erika Check Hayden, The automated lab Nature. ,vol. 516, pp. 131- 132 ,(2014) , 10.1038/516131A