DETECTION AND GENETIC RELATIONSHIP OF DENGUE VIRUS SEQUENCES IN SEVENTEEN-YEAR-OLD PARAFFIN-EMBEDDED SAMPLES FROM CUBA
作者： C A Sariol , G Kouri , E Arteaga , M G Guzman , J L Pelegrino
DOI: 10.4269/AJTMH.1999.61.994
关键词:
摘要: This study describes the use of reverse transcriptase-polymerase chain reaction (RT-PCR) to generate dengue 2 amplicons from paraffin-embedded autopsy tissues collected in Cuba 17 years ago. The presumptive diagnoses had been made only by clinical evolution without serologic confirmation. confirms once again that virus was directly associated with fatal cases children and illustrates potential RT-PCR for retrospective diagnosis after death. A close similarity genomic sequences RNA detected tissue samples those Cuban strains previously investigated appropriate classification etiologic agent 1981 hemorrhagic fever epidemic.
paperity.org参考文章(21)
F P Pinheiro, S J Corber, Global Situation of Dengue and Dengue Haemorrhagic Fever, and Its Emergence in the Americas World health statistics quarterly. Rapport trimestriel de statistiques sanitaires mondiales. ,vol. 50, pp. 161- 169 ,(1997)
Gustavo P Kouri, María G Guzmán, J Ramon Bravo, C Triana, None, Dengue haemorrhagic fever/dengue shock syndrome: lessons from the Cuban epidemic, 1981. Bulletin of The World Health Organization. ,vol. 67, pp. 375- 380 ,(1989)
R S Lanciotti, C H Calisher, D J Gubler, G J Chang, A V Vorndam, Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. Journal of Clinical Microbiology. ,vol. 30, pp. 545- 551 ,(1992) , 10.1128/JCM.30.3.545-551.1992
Gustavo Kouri, Reemergence of Dengue in Cuba: A 1998 Epidemic in Santiago de Cuba Emerging Infectious Diseases. ,vol. 4, pp. 85- 88 ,(1998) , 10.3201/EID0401.980111
JR Bravo, MG Guzman, GP Kouri, Why dengue haemorrhagic fever in Cuba? I. Individual risk factors for dengue haemorrhagic fever/dengue shock syndrome (DHF/DSS) Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 81, pp. 816- 820 ,(1987) , 10.1016/0035-9203(87)90041-1
Rebeca Rico-Hesse, Molecular evolution and distribution of dengue viruses type 1 and 2 in nature. Virology. ,vol. 174, pp. 479- 493 ,(1990) , 10.1016/0042-6822(90)90102-W
Daoroong Kangwanpong, Natth Bhamarapravati, Helen L. Lucia, Diagnosing dengue virus infection in archived autopsy tissues by means of the in situ PCR method: a case report Clinical and Diagnostic Virology. ,vol. 3, pp. 165- 172 ,(1995) , 10.1016/0928-0197(94)00032-P
Helen L. Lucia, Daoroong Kangwanpong, Identification of Dengue virus-infected cells in paraffin-embedded tissue using in situ polymerase chain reaction and DNA hybridization Journal of Virological Methods. ,vol. 48, pp. 1- 8 ,(1994) , 10.1016/0166-0934(94)90083-3
Rebeca Rico-Hesse, Lisa M. Harrison, Rosa Alba Salas, Duilia Tovar, Ananda Nisalak, Celso Ramos, Jorge Boshell, Maria Teresa R. de Mesa, Rita M.R. Nogueira, Amélia Travassos da Rosa, Origins of dengue type 2 viruses associated with increased pathogenicity in the Americas. Virology. ,vol. 230, pp. 244- 251 ,(1997) , 10.1006/VIRO.1997.8504
Young S. Hahn, Ricardo Caller, Tim Hunkapiller, Joel M. Dalrymple, James H. Strauss, Ellen G. Strauss, Nucleotide sequence of dengue 2 RNA and comparison of the encoded proteins with those of other flaviviruses Virology. ,vol. 162, pp. 167- 180 ,(1988) , 10.1016/0042-6822(88)90406-0