Let's get fISSical: fast in silico synchronization as a new tool for cell division cycle analysis.

摘要: Cell cycle progression is a question of fundamental biological interest. The coordinated duplication and segregation all cellular structures organelles however an extremely complex process, one which remains only partially understood even in the most intensively researched model organisms. Trypanosomes are unusual position this respect - they both outstanding systems for questions eukaryotic cell biology, pathogens that causative agents three neglected tropical diseases. As failure to successfully complete division will be deleterious or lethal, analysis relevance basic biology drug design efforts. experimentally challenging, as phenotypes associated with it hypothesis-driven therefore biased. Current methods labour-intensive, synchronization difficult unreliable. Consequently, there exists need applied trypanosome global, unbiased, standardized high-throughput progression. In review, requirements practical computational such system considered compared existing techniques analysis.