Screening for the rice blast resistance gene Pi-ta using LNA displacement probes and real-time PCR

摘要: Single nucleotide polymorphisms (SNPs) will become a molecular breeding tool of increasing significance as growing range SNP data becomes available. In order for these markers to be incorporated into programs, simple, high throughput and low cost detection methods need We have developed such an assay using LNA containing displacement probes the Pi-ta gene, important blast resistance gene in rice. This gave superior performance comparison with TaqMan MGB was able accurately identify presence frequency genotypes artificially created mixed samples. The ability pool samples screening purposes offers potential significantly increase reduce per sample cost, particularly alleles.