Sensitivity of Sertoli and Leydig cells to xenobiotics in in vitro models.

摘要: Different chemicals are known to cause testicular damage in the human male and experimental animals. However, ability assess potential mechanism of action leading chemically-induced men has been hampered by a lack good predictive models. Although many these were found impair reproductive capacity various laboratory animals, only some have caused men. Mammalian spermatogenesis takes places within avascular seminiferous tubules testis. Specialized tight junctions, which form between adjacent Sertoli cells at time puberty, divide tubular space into basal adluminal compartments, create "blood-testis" barrier that restricts passage substances ions from circulation. Thus, completion meiosis post-meiotic germ cell differentiation, take place compartment, isolated circulating unable cross blood-testis barrier. It seems feasible, therefore, induced toxicants may be mediated through other testis such as Sertoli, peritubular, or Leydig cells. A recently developed two-compartment system for culture can simulate, degree, normal physiologic conditions. In principle, mammalian testes cultured on permeable support (that is millipore filter) two fluid compartments. They highly polarized epithelial layer with characteristic junctions restrict analogy We believe this provides an excellent vitro model determining to: a) alter permeability barrier, b) secretory function cells, c) affect their viability, all could indirectly utilized examining effects cadmium chloride (CdCl2) toxic The toxicity was investigated perfusion cultures