A cold-regulated Arabidopsis gene encodes a polypeptide having potent cryoprotective activity.
作者： Chentao Lin , Michael F. Thomashow
DOI: 10.1016/S0006-291X(05)80304-3
关键词:
摘要: Summary We previously showed that cor15 , a cold-regulated gene from Arabidopsis thaliana encodes 15 kDa polypeptide designated COR15. Here we report COR15 has potent cryoprotective activity in an vitro cryoprotection assay. Specifically, was very effective protecting the cold-labile enzyme lactate dehydrogenase against freeze-inactivation; on concentration basis, it about 10 6 times more than sucrose and 2 –10 3 other proteins including bovine serum albumin. The possible role of cold acclimation is discussed.
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sci-hub.se 参考文章(17)
C. J. Weiser, Cold Resistance and Injury in Woody Plants: Knowledge of hardy plant adaptations to freezing stress may help us to reduce winter damage. Science. ,vol. 169, pp. 1269- 1278 ,(1970) , 10.1126/SCIENCE.169.3952.1269
J.C. Lee, S.N. Timasheff, The stabilization of proteins by sucrose. Journal of Biological Chemistry. ,vol. 256, pp. 7193- 7201 ,(1981) , 10.1016/S0021-9258(19)68947-7
Ravindra K. Hajela, David P. Horvath, Sarah J. Gilmour, Michael F. Thomashow, Molecular Cloning and Expression of cor (Cold-Regulated) Genes in Arabidopsis thaliana Plant Physiology. ,vol. 93, pp. 1246- 1252 ,(1990) , 10.1104/PP.93.3.1246
Toru Tamiya, Nobuo Okahashi, Ryozo Sakuma, Toshifumi Aoyama, Takayuki Akahane, Juichiro J. Matsumoto, Freeze denaturation of enzymes and its prevention with additives. Cryobiology. ,vol. 22, pp. 446- 456 ,(1985) , 10.1016/0011-2240(85)90156-7
Peter L. Steponkus, Daniel V. Lynch, Freeze/thaw-induced destabilization of the plasma membrane and the effects of cold acclimation. Journal of Bioenergetics and Biomembranes. ,vol. 21, pp. 21- 41 ,(1989) , 10.1007/BF00762210
Chentao Lin, Wei Wen Guo, Everett Everson, Michael F. Thomashow, Cold Acclimation in Arabidopsis and Wheat : A Response Associated with Expression of Related Genes Encoding `Boiling-Stable' Polypeptides Plant Physiology. ,vol. 94, pp. 1078- 1083 ,(1990) , 10.1104/PP.94.3.1078
H.G. Volger, U. Heber, Cryoprotective leaf proteins. Biochimica et Biophysica Acta. ,vol. 412, pp. 335- 349 ,(1975) , 10.1016/0005-2795(75)90048-3
Donald Greiff, Richard T. Kelly, Cryotolerance of enzymes Cryobiology. ,vol. 2, pp. 335- 341 ,(1966) , 10.1016/S0011-2240(66)80122-0
KEIJI SHIKAMA, TSAO YAMAZAKI, Denaturation of Catalase by Freezing and Thawing Nature. ,vol. 190, pp. 83- 84 ,(1961) , 10.1038/190083A0
Timothy J. Close, Alexander A. Kortt, Peter M. Chandler, A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Molecular Biology. ,vol. 13, pp. 95- 108 ,(1989) , 10.1007/BF00027338