Regulatory role of osteopontin in malignant transformation of endometrial cancer
作者： Sabarish Ramachandran , Kun-Young Kwon , So-Jin Shin , Sang-Hoon Kwon , Soon-Do Cha
DOI: 10.1007/S11033-012-2436-8
关键词:
摘要: Osteopontin (OPN) involves in the tumor-promoting or metastasis human endometrial cancer. Depletion of OPN gene expression cancer cells was significantly decreased cell viability and undergo apoptotic death. The status THESC, RL95, Hec1A Ishikawa lines were analyzed by RT-PCR western blot. After OPN-siRNA transfection, mRNA protein levels determined cells. Cell proliferation cycle distribution observed MTT flow cytometry analysis. DNA fragmentation assay used to measure apoptosis. migration assessed wound healing assay. (Hec1A cells) reproducibly changed their ability proliferation. Concomitant changes seen binding surface receptors, cycle-regulatory genes, invasion colony formation nature tumor Decreased colonizing potential absence reversed presence recombinant OPN. Inhibition anchorage-independent growth metabolic inhibitors PI3K, Src integrin signaling cascades, which ameliorated exogenously added Our result showed role cancer, particular on malignancy-promoting aspects that may pave way for new approaches clinical management
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