Formação de biofilme em aço inoxidável por Aeromonas hydrophila e Staphylococcus aureus usando leite e diferentes condições de cultivo
作者： Cleube Andrade Boari , Mariana Pereira Alves , Victor Maximiliano Reis Tebaldi , Taciana Villela Savian , Roberta Hilsdorf Piccoli
DOI: 10.1590/S0101-20612009000400029
关键词:
摘要: The aim of this research was to verify the capability biofilm formation on stainless steel by Aeromonas hydrophila and Staphylococcus aureus using milk different conditions cultivation. variables consisted in mono multi-species cultivation these microorganisms temperatures 4, 7 18 °C. Containers containing 1000 mL milk, population density 105 CFU.mL-1 each microorganism, ten suspended chips AISI 304 (10 × 20 mm) were used seal up storage, under 60 rpm agitation for 10 days. analyses conducted every 48 hours. Sessile cells A. S. enumerated plating m-Aeromonas selective agar Baird-Parker agar. Complementary studies included generation time calculation, enumeration planktonic cells, visualization scanning electron microscopy. aureus, mono-cultivation, formed at °C At 4 observed attachment. presence reduced performance aureus. In condition, 18 °C. all conditions.
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