Phorbol Ester- and Growth Factor-Induced Growth Hormone (GH) Receptor Proteolysis and GH-Binding Protein Shedding: Relationship to GH Receptor Down-Regulation1

摘要: GH signals by interacting with receptor (GHR). A substantial fraction of circulating complexes GH-binding protein (GHBP), which corresponds to the GHR extracellular domain. GHBP is generated 1) alternative splicing a common precursor messenger RNA encode secreted (the source vast majority in rodents); and 2) proteolysis cell-associated shedding (a mechanism operative rabbits humans). We previously observed that phorbol ester (PMA)-induced activation kinase C (PKC) causes metalloprotease-mediated human IM-9 lymphocytes. now demonstrate PMA-induced hydroxamate (IC3)-inhibitable were also detected murine 3T3-F442A 3T3-L1 preadipocytes Chinese hamster ovary (CHO) cells stably expressing rabbit (rbGHR), although degree was much smaller for than or GHRs. i...