Detection of Human Papillomavirus in Human Focal Cortical Dysplasia Type IIB

摘要: Objective: Focal cortical dysplasia type IIB (FCDIIB) is a sporadic developmental malformation of the cerebral cortex highly associated with pediatric epilepsy. Balloon cells (BCs) in FCDIIB exhibit constitutive activation mammalian target rapamycin complex 1 (mTORC1) signaling pathway. Recently, high-risk human papillomavirus 16 oncoprotein E6 was identified as potent activator mTORC1 signaling. Here, we test hypothesis that HPV16 present specimens. Methods: HPV16 protein expression assayed by immunohistochemistry specimens (n = 50) and control brain 36). DNA polymerase chain reaction (PCR) situ hybridization; mRNA reverse transcriptase PCR. transfected into fetal mouse brains utero electroporation to effects on development. Results: HPV16 robustly expressed all BCs, but not regions without BCs or tissue including normal brain, lymphoblasts, fibroblasts, tubers, U87 glioma cells. colocalized phosphoactivated S6 protein, known substrate. were detected representative cortex, confirmed sequencing. Transfection caused focal association enhanced signaling. Interpretation: Our results indicate new between demonstrate for first time brain. We propose novel etiology based during development. ANN NEUROL 2012;72:881–892