Genome signatures of colon carcinoma cell lines.

摘要: In cancer biology, cell lines are often used instead of primary tumors because their widespread availability and close reflection the in vivo state. Cancer is a genetic disease, commonly caused by small- large-scale DNA rearrangements. Therefore, it essential to know genomic profiles tumor enable correct efficient use as experimental tools. Here, we present comprehensive study 20 colon combining conventional karyotyping (G-banding), comparative hybridization (CGH), multicolor fluorescence situ (M-FISH). Major differences between microsatellite instability (MSI) chromosome (CIN) shown; CIN exhibited complex karyotypes involving many chromosomes (mean: 8.5 copy number changes), whereas MSI showed considerably fewer aberrations 2.6). The 3 techniques complement each other provide detailed picture numerical structural chromosomal changes that characterize cells. 7 (Colo320, EB, Fri, IS2, IS3, SW480, V9P) here completely karyotyped for first time and, among these, 5 have not previously been cytogenetically described. By hierarchical cluster analysis, show representative models carcinomas at genome level. We also an model progression, including (IS1, IS3) established from carcinoma, its corresponding liver- peritoneal metastasis same patient. To address question clonality, compared common grown 2 laboratories. Finally, all our results with published CGH data colorectal lines. conclusion, large variation complexity highlights importance reference investigators engaged functional studies using these research