Association of p53 accumulation with TP53 mutations, loss of heterozygosity at 17p13, and DNA ploidy status in 273 colorectal carcinomas.

摘要: The aim of this study was to establish an experimentally based cutoff level for assessing p53 immunoreactivity in colorectal tumors. accumulation protein 273 tumors correlated with previously obtained data on TP53 mutation and loss heterozygosity at two 17p13 loci the same monoclonal antibody PAb 1801 used staining, results by immunohistochemistry immunoblotting were similar. Mutation analyses exons 5-8 performed using constant denaturant gel electrophoresis followed sequencing. There no statistically significant differences any measured gene alteration between group without p53-positive nuclei (n = 83) 1.3) indicates that DNA hyperdiploid constitute a separate developmental entity different from gross aneuploidy.