Improving draft genome contiguity with reference-derived in silico mate-pair libraries.
作者： José Horacio Grau , Thomas Hackl , Klaus-Peter Koepfli , Michael Hofreiter
DOI: 10.1093/GIGASCIENCE/GIY029
关键词:
摘要: Background Contiguous genome assemblies are a highly valued biological resource because of the higher number completely annotated genes and genomic elements that usable compared to fragmented draft genomes. Nonetheless, contiguity is difficult obtain if only low coverage data and/or distantly related reference available. Findings In order improve contiguity, we have developed Cross-Species Scaffolding—a new pipeline imports long-range distance information directly into de novo assembly process by constructing mate-pair libraries in silico. Conclusions We show how metrics gene prediction dramatically with our assembling two primate genomes solely based on ∼30x shotgun sequencing data.
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