Measurement of ROS homeostasis in isolated mitochondria.

摘要: In this chapter, we describe the currently most advanced methods applied for quantitative assessment of ROS homeostasis inside mitochondrion. These techniques are particular interest in field oxidative stress. After discussing importance quantifying mitochondrial homeostasis, three major aspects phenomenon and pertinent methodologies detection delineated detail. First important methods, based on fluorimetric or spectrophotometric approaches, described. Elimination generated mitochondrion is another crucial mechanism that also needs to be quantified accurately estimate antioxidant capacity mitochondria under specific conditions. Since generation elimination manifest concert, there exist independent estimation net effect. Such a sensitive biochemical marker aconitase, citric acid cycle enzyme which greatly ROS. We two procedures precise determination aconitase activity. A few auxiliary good practices having relevance successful accomplishment more delicate approaches mentioned. All other relevant technical considerations including advantages/disadvantages various common artifacts discussed.