The quantitative measurement of H2O2 generation in isolated mitochondria.
作者： Gustavo Barja
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摘要: Adequate methods to measure the rate of mitochondrial oxygen radical generation are needed since radicals involved in many pathologies. A fluorometric method appropriate H2O2 intact mitochondria is described. Just after isolation functional from fresh tissues, rates kinetically measured by fluorometry presence homovanillic acid and horseradish peroxidase. The specific for sensitive enough assay respiratory substrate without inhibitors chain. Simultaneous measurement consumption allows calculation free leak: percentage electrons out sequence which reduce along chain instead reducing water at terminal cytochrome oxidase. shows instantaneous response H2O2. This makes it study quick effects different modulators on production. Its application localization sites where caloric restriction decreases heart
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