Conjugated Linoleic Acid (t-10, c-12) Reduces Fatty Acid Synthesis de Novo, but not Expression of Genes for Lipid Metabolism in Bovine Adipose Tissue ex Vivo
作者： Seong Ho Choi , David T. Silvey , Bradley J. Johnson , Matthew E. Doumit , Ki Yong Chung
DOI: 10.1007/S11745-013-3869-0
关键词:
摘要: We hypothesized that exogenous fatty acids, and especially or 18:2 trans-10, cis-12 conjugated linoleic acid (CLA), would decrease adipogenic lipogenic gene expression de novo biosynthesis in intramuscular (i.m.) subcutaneous (s.c.) adipose tissues. Fresh i.m. s.c. tissues were collected from the longissimus thoracis muscle of Angus steers at 12, 14, 16 months age (n = 4 per time point). Adipose tissue explants incubated duplicate for 48 h with 40 μM α-linolenic (ALA), oleic, stearic, trans-vaccenic, CLA. Adipocyte size, acetate glucose incorporation into acids vitro mRNA levels C/EBPβ, CPT1β, GPR43,PPARγ, PRKAA1 (AMPKα) SCD1 measured following incubations. SCD1gene greater (P 0.10). Thus, CLA depressed but neither nor other significantly affected expression.
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