Two new acute lymphoblastic leukemia cell lines with early B-cell phenotypes

摘要: Two leukemic cell lines (697 and 207) were established from bone marrow cells obtained children with ALL in relapse. These positive for the common-ALL antigen (CALLA), HLA-DR (i.e., Ia-like) antigen, cytoplasmic surface IgM heavy chains. The negative other immunoglobulin chains light had elevated levels of terminal deoxynucleotidyl transferase enzyme expressed antigens found on normal myeloid-macrophage (MMA) natural killer (HNK-1). A minority line 207 T-1, T-6, Leu-1 as detected by monoclonal antibodies. Line 697 was Epstein-Barr virus (EBV), while did not possess EBV. carried a marker chromosome (identified translocation between chromosomes 7 19), which also patient's fresh cells. origin further indicated their morphological, cytochemical, immunologic similarity to patients' Phenotypically, both appear be arrested transitional stage development pre-B B express usually non-B-cell lineages.