Biomimetic synthesis of Mg-substituted hydroxyapatite nanocomposites and three-dimensional printing of composite scaffolds for bone regeneration.
作者： Shangsi Chen , Yufei Shi , Xin Zhang , Jun Ma
DOI: 10.1002/JBM.A.36757
关键词:
摘要: In this study, we have successfully fabricated magnesium (Mg) substituted hydroxyapatite nanocomposites (Mg-HA) by utilizing type I collagen (COL I) and citric acid (CA) through a bitemplate-induced biomimetic mineralization approach. The obtained composite nanoparticles were subsequently mixed with chitosan (CHI) gelatin (Gel) to prepare porous scaffolds interconnected structures three-dimensional (3D) printing technique. Mg-HA powders characterized. results showed that the substitution of Mg for Ca ions reduced crystallinity HA crystals, but did not significantly affect size structure nanocomposites. morphology turned smoother compared substitution. Furthermore, biocompatibility was evaluated metal ion release, cell attachment, proliferation, differentiation MC3T3-E1 cells. According results, as more Ca2+ Mg2+ , released from samples pH in cultured medium acidic. It suggested presented higher proliferation rate, increased expression alkaline phosphatase (ALP) activity osteogenic related gene, including osteocalcin (OCN), runt-related transcription factor 2 (RUNX2), COL I. Therefore, it indicated 3D printed excellent bioactivity potential candidate bone tissue engineering.
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