作者: Paul H Dear , Angelika Hertha Elisabeth Daser
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摘要: The present invention provides a method for counting the absolute copy number of nucleic acid sequence in cell, which comprises following steps: (i) dividing lysate cell or sample into plurality aliquots: (ii) providing conditions suitable amplification each aliquot: (iii) aliquots was amplified step and directly deducing cell. may be used to count chromatid number, example investigate ploidy such as an oocyte embryo-derived