Phagocytosis of staphylococci biofilms by polymorphonuclear neutrophils: S. aureus and S. epidermidis differ with regard to their susceptibility towards the host defense.

摘要: Bacteria organized in biofilms are a common cause of relapsing or persistent infections. In patients receiving orthopedic implants, such as endoprostheses osteosynthesis materials, Staphylococcus aureus and S. epidermidis prevalent it is widely assumed that bacteria not only relatively resistant towards antibiotics biocides, but also host defense mechanisms. context, we addressed the question how polymorphonuclear neutrophils (PMN), "first line defense" against bacterial infection, interact with generated vitro. By time-lapse video microscopy, observed migration PMN biofilms. case aureus, moved across biofilm took up they along. On epidermidis, contrast, were rather immobile, phagocytosis was limited to immediate vicinity. labeling within H-thymidine found more sensitive attack than epidermidis. Following either strain, underwent apoptosis, dogma, induces programmed cell-death order prevent spilling bactericidal cytotoxic entities. conclusion, inherently protected by phagocytic cells; their sensitivity, however, varies among strains, presumably due properties extracellular matrix affecting motility on film.