The Self-Association of Chymotrypsinogen A

摘要: The self-association of chymotrypsinogen A at pH 8.1 and ionic strength 0.021 has been examined by means sedimentation equilibrium. data have analyzed several methods results compared. Application the older available in literature indicate that concentration dependence molecular weight may be well accounted for terms an indefinite association which consecutive stepwise constants are equal second virial coefficient is small positive. A new method developed, based on series expansion, selects values set statistically significant give optimum least squares fit data. this indicates extends to tetramer. The best model process a similar magnitudes upper limit undefined. This essentially equivalent model. Frontal analysis using gel filtration gives compatible with above model.