Production and subsequent second-order decomposition of protein disulfide anions

摘要: Abstract By a technique of radiation chemistry, one can convert small groups on protein (surface disulfide bonds) into reactive centers (disulfide anion radicals) in less than 100 μs. The different molecules then proceed to react with each other and are destroyed. radical is formed by electron transfer from transient CO2− generated pulse radiolysis (Adams et al., 1972). formation decay the were studied monitoring its absorption at 410 nm. reliable qualitative but not quantitative test for presence surface groups. area this center very compared total protein. Nevertheless rate reaction 13 proteins ranges 2% 50% diffusion controlled 3 × 109 m −1 s−1. Billiard ball type collisions during which briefly touch do account these fast rates. We conclude that collision has substantial duration. A radially symmetric potential holds together allows them roll others' surfaces. model Sole & Stockmayer (1973) be applied reaction, showing duration typically 10 This conclusion, valid 12 studied, seems general. Whenever two approach other, attractive forces hold long enough mutual rotation bring contact, necessary sometimes sufficient condition their reaction.