Oxygen requirement and energy relations of itaconic acid fermentation by Aspergillus terreus NRRL 1960

摘要: The effect of interrupting aeration on itaconic acid fermentation by A. terreus NRRL 1960 has been studied. Under the conditions used, stopping for 5 min led to a complete cessation production, which was only slowly restored after 24 h when resumed. After 5-min break in and presence 0.1 mM cycloheximide no formed even 3 days. It seems that, upon oxygen shortage, rapid destruction itaconic-acid-producing mechanism takes place, is aerobically slow process involving protein synthesis. Itaconic also effectively stopped metabolic inhibitors ATP formation, pointing need biochemical energy maintaining fermentation. possibly needed maintain proper physiological (i.e. near neutral) pH inside cells, counteracting produced low external (below 2.0). Inhibitors plasma membrane ATPase have This indicates that might be impermeable H+ rather involved transport out cell. suggested insufficient may leads production which, turn, damage machinery process.