Retraction. Helicobacter pylori-induced interleukin-12 p40 expression.

摘要: Interleukin-12 (IL-12) is a heterodimeric cytokine produced by antigen-presenting cells that promotes the development of T-helper lymphocyte 1 (Th1). Chronic gastritis induced Helicobacter pylori considered Th1-mediated process. IL-12 levels in gastric biopsy samples H. pylori-infected patients are higher than those uninfected individuals, but cellular source remains elusive. staining was detected mucosal epithelial cells, lymphocytes, and macrophages specimens with pylori-positive gastritis. Therefore, we investigated p40 mRNA induction T cells. Although cag pathogenicity island (PAI)-positive expression, an isogenic mutant PAI failed to induce it both cell types. Supernatants from cultures VacA expression not The activation promoter mediated through NF-κB. transfection IκB kinase NF-κB-inducing dominant-negative mutants inhibited pylori-induced activation. Inhibitors NF-κB, phosphatidylinositol 3-kinase, p38 mitogen-activated protein kinase, Hsp90 suppressed pylori- VacA-induced expression. results indicate induces kinase. also crucial regulator In addition PAI, might be relevant Th1-polarized response only