Incomplete transformation of rat cells by a small fragment of adenovirus 12 DNA

摘要: Rat cells (3Y1) were transformed by the Bpa-H fragment (4.5%, left end) of adenovirus 12 (Ad 12) DNA, cloned, and cell lines (HY1 to HY7 cells) established. Most sequence BpaI-H Ad DNA was present in HY1 HY2 cells. The properties HY (HY1, HY2, HY7), GY1 (a rat line HindIII-G DNA), CY1 EcoRI-C fragment), WY3 whole DNA) examined. morphology growth Eagle's minimal essential medium with 10% fetal calf serum HY1, HY5, similar cells, showing phenotype. However, following intermediate between untransformed cells: (1) 2% serum; (2) colony formation soft agar culture; (3) cellular synthesis Methocel medium; (4) tumor after transplantation into rats. T antigens not detected immunofluorescence. Viral antigen could be extract complement fixation test. These results indicate that are incomplete significance transformation is discussed.