Inducible Expression of Erythroid-Specific Mouse Glycophorin Gene Is Regulated by Proximal Elements and Locus Control Region-Like Sequence.

摘要: Cis-acting elements of the gene for mouse glycophorin, an erythroid-specific membrane glycoprotein, were determined by transient and stable transfection assays using murine erythroleukemia (MEL) cells. proximal to transcription start site can be separated into basal promoter (-1 191 bp) distal element (-133 -92). The contained GGTGG GATA motifs GATA-1 NF-E2 motifs. Deletion analysis its neighboring sequence DNase-I footprinting/EMSA (electrophoretic mobility shift assay) indicated that induced nuclear factor binding may required expression during MEL cell differentiation dimethyl sulfoxide treatment. was also shown essential activity. An approximately 400 bp far upstream region (-1325 -948bp) containing GGGTGG, showed no enhancing activity when this examined assay, but it did show enhancement differentiation-specific in assay. glycophorin have a function similar locus control (LCR) human beta-globulin cluster.