Large molecule bioanalysis using Q-TOF without predigestion and its data processing challenges
作者： Suma Ramagiri , Fabio Garofolo
DOI: 10.4155/BIO.12.10
关键词:
摘要: Background: Recent developments in LC–MS have turned it into a viable and valid alternative to ligand-binding assays. Large molecule bioanalysis by is generally performed tryptic digestion, purification detection of one or more small signature peptides. High-resolution MS instruments offer quantification intact proteins peptides are able increase the selectivity, while maintaining sensitivity. Results: Unlike multiple reaction monitoring assays, several factors affecting data processing were presented optimal parameters consider during method building also demonstrated. MUC5AC-13 (MW 1709.8 Da), human hepcidin/LEAP-1 2797.4 porcine calcitonin 3604 Da) chicken lysozyme 14.3 kDa) selected as model compounds possibility peptide protein quantification, without was Conclusion: Selectivity sensitivity improved using different scan modes, such TOF-MS an...
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